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The 10X Genomics Chromium Single Cell 3’ GEM Library and Gel Bead Kit v3 enables simultaneous library preparation of hundreds to thousands of individual cells for 3’ digital gene expression profiling analysis. Cell suspensions (containing 800 to 16,000 cells per sample) are loaded into a Chromium Chip B along with partitioning oil, reverse.
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Our standard gene expression assay is run using the 5' 10X Genomics method, and the VDJ and Feature Barcode library preps are available as add-on.

Answer: Multiome Gene Expression libraries can be pooled with other dual-indexed gene expression libraries. Multiome ATAC libraries can be pooled with standalone ATAC libraries only on certain Illumina sequencers like MiSeq, HiSeq 2000/2500, NovaSeq 6000 (v1 reagents) sequencers and using 16bp for the i5 read. Although typically, Multiome ATAC.
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  • マルチプレックス法は、NGSのキャパシティ最大化に役立つ手法で、単一のフローセルでマルチプルライブラリーを解析することで(多くの場合、数千)、コストの削減が可能になります。. DNAシークエンシングコストが低くなり、リプロダクティブ・ヘルス ...
  • A typical scRNA-seq workflow includes most of the following steps: 1) isolation of single cells, 2) cell lysis while preserving mRNA, 3) mRNA capture, 4) reverse transcription of primed RNA into complementary DNA (cDNA), 5) cDNA amplification, 6) preparation of cDNA sequencing library, 7) pooling of sequence libraries, 8) use of bio-informatic ...
  • 1. 2. 3. For specifics on total amount and volume required, please see RNA and DNA library preparation options in the charts below. Submit all samples in 1.5ml eppendorf tubes, clearly labeled ON TOP of tube with sample name and user name/initials. Label the SIDE of tube with the date. Sample name on tube and iLab request form should match.